Plant tissue culture, development and biotechnology / edited by Robert N. Trigiano, Dennis J. Gray.

Includes bibliographical references and index.

INTRODUCTION
Introduction to plant tissue culture, development and biotechnology, D.J. Gray and R.N. Trigiano

SUPPORTING METHODOLOGIES AND GENERAL CONCEPTS
Getting started with tissue culture — media preparation, sterile technique and laboratory equipment, C.A. Beyl
Laboratory Exercise: Nutrition of cell and organ cultures, J. Capponetti and R.N. Trigiano
Plant growth regulators in plant tissue culture and development, C.A. Beyl
Elements of in vitro research, M.E. Compton
Concept/Laboratory Exercise: Proper use of microscopes, D.T. Webb
Concept/Laboratory Exercise: Plant histological techniques, R.N. Trigiano, D.J. Gray, K.R. Malueg, K.A. Pickens, Z.-M. Cheng, and E.T. Graham
A brief introduction to plant anatomy and morphology, R.N. Trigiano, J.A. Franklin and D.J. Gray
Seed development and germination, F. Chen, R.C. Martin, S. Song and H. Nonogaki
Concept/Labortory Exercise: Molecular tools for studying plant genetic diversity, T. Rinehart, X. Wang, R.N. Trigiano, N.R. Rowland and R.E. DeVries
Molecular approaches to the study of plant development, A. von Arnim and B.-H. Kim

PROPAGATION AND DEVELOPMENT CONCEPTS
Propagation from meristematic tissue -- shoot culture, M.E. Kane
Laboratory Exercise: Micropropagation of Sygonium by shoot culture, M.E. Kane
Laboratory Exercise: Micropropagation and in vitro flowering of rose, M.E. Kane, T. Johnson and P. Kauth
Laboratory Exercise: Micropropagation of potato by node culture and microtuber production, M.E. Kane
Commercial laboratory production, G.R.L. Suttle
Detection and elimination of microbial endophytes and prevention of contamination in plant tissue cultures, A.C. Cassells
Laboratory Exercise: Culture indexing for bacterial and fungal contaminants, M.E. Kane, P. Kauth and T. Johnson
Propagation from nonmeristematic tissues -- Organogenesis, R. Geneve
Developing a molecular understanding of in vitro and in planta shoot organogenesis, L. Meng, S. Zhang and P.G. Lemaux
Laboratory Exercise: Direct shoot organogenesis from chrysanthemum and african violet leaves, R.N. Trigiano, L.M. Vito, M.T. Windham, S. Boggus, and D. Hadziabdic
Propagation from nonmeristematic tissues - nonzygotic embryogenesis, D.J. Gray
Developmental and molecular aspects of nonzygotic (somatic) embryogenesis, X. Yang and X. Zhang
Laboratory Exercise: Embryogenic callus and suspension cultures from leaves of orchardgrass, D.J. Gray, R.N. Trigiano and B.V. Conger
Laboratory Exercise: Direct non-zygotic embryogenesis from leaves of cineraria, R.N. Trigiano, M.C. Scott, and K.R. Malueg

CROP IMPROVEMENT TECHNIQUES
Protoplasts – an increasingly valuable tool in plant research, J.W. Grosser and A.A. Omar
Laboratory Exercise: Demonstration of principles of protoplast isolation using chrysanthemum and orchardgrass leaves, R.N. Trigiano
Laboratory Exercise: Isolation, culture and fusion of tobacco and potato protoplasts, R.E. Veilleux and M.E. Compton
Haploid cultures, D. Hadziabdic, P.A. Wadl and S.M. Reed
Laboratory Exercise: Haploid plants from tobacco and potato anthers, P.A. Wadl, D. Hadziabdic and S.M. Reed
Concept/Laboratory Exercise: Embryo rescue, T. Eeckhaut , K. Van Laere and J. Van Huylenbroeck
Promoters and gene expression regulation, Z.T. Li and D.J. Gray
Genetic engineering technologies, Z.T. Li, S.A. Dhekney and D.J. Gray
Concept/Laboratory Exercise: Transformation of plant meristems, J.H. Gould
Laboratory Exercise: Genetic transformation of chysanthemum and tobacco using Agrobacterium tumefaciens, M.M. Young, L. Padegimas, N.A. Reichert and R.N. Trigiano
Laboratory Exercise: Genetic transformation of tobacco and production of transgenic plants, S.A. Dhekney, Z.T. Li and D.J. Gray
Genetically modified plant controversies: Sensational headlines versus pragmatic research, H.A. Richards, L.C. Hudson, M.D. Halfhill and C.N. Stewart, Jr.
Cryopreservation of plant cells, tissues and organs, B.M. Reed, M.N. Normah and S.V. Kushnarenko
Laboratory Exercise: Vitrification as a method to cryopreserve shoot tips, S.V. Kushnarenko, B.M. Reed and M.N. Normah
Laboratory Exercise: Cryopreservation of orthodox and recalcitrant seed, M.N. Normah, W.K. Choo, S.V. Kushnarenko and B. Reed
Plant Biotechnology for the Production of Natural Products, A. Kirakosyan, E. M. Seymour and P. Kaufman
Laboratory Exercise: Pigment production in Ajuga cell culture, M.A. Lila and R.B. Rogers
Variations in tissue culture, R.M. Skirvin and M. Norton

THE BUSINESS OF BIOTECHNOLOGY
Biotechnology entrepreneurship in the 21st century: From bench to bag, D.W. Altman
Intellectual property protection for plants, C. Eisenshank