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INTRODUCTION<br/>Introduction to plant tissue culture, development and biotechnology, D.J. Gray and R.N. Trigiano<br/><br/>SUPPORTING METHODOLOGIES AND GENERAL CONCEPTS<br/>Getting started with tissue culture — media preparation, sterile technique and laboratory equipment, C.A. Beyl<br/>Laboratory Exercise: Nutrition of cell and organ cultures, J. Capponetti and R.N. Trigiano<br/>Plant growth regulators in plant tissue culture and development, C.A. Beyl<br/>Elements of in vitro research, M.E. Compton<br/>Concept/Laboratory Exercise: Proper use of microscopes, D.T. Webb<br/>Concept/Laboratory Exercise: Plant histological techniques, R.N. Trigiano, D.J. Gray, K.R. Malueg, K.A. Pickens, Z.-M. Cheng, and E.T. Graham<br/>A brief introduction to plant anatomy and morphology, R.N. Trigiano, J.A. Franklin and D.J. Gray<br/>Seed development and germination, F. Chen, R.C. Martin, S. Song and H. Nonogaki<br/>Concept/Labortory Exercise: Molecular tools for studying plant genetic diversity, T. Rinehart, X. Wang, R.N. Trigiano, N.R. Rowland and R.E. DeVries<br/>Molecular approaches to the study of plant development, A. von Arnim and B.-H. Kim<br/><br/>PROPAGATION AND DEVELOPMENT CONCEPTS<br/>Propagation from meristematic tissue -- shoot culture, M.E. Kane<br/>Laboratory Exercise: Micropropagation of Sygonium by shoot culture, M.E. Kane<br/>Laboratory Exercise: Micropropagation and in vitro flowering of rose, M.E. Kane, T. Johnson and P. Kauth<br/>Laboratory Exercise: Micropropagation of potato by node culture and microtuber production, M.E. Kane<br/>Commercial laboratory production, G.R.L. Suttle<br/>Detection and elimination of microbial endophytes and prevention of contamination in plant tissue cultures, A.C. Cassells<br/>Laboratory Exercise: Culture indexing for bacterial and fungal contaminants, M.E. Kane, P. Kauth and T. Johnson<br/>Propagation from nonmeristematic tissues -- Organogenesis, R. Geneve<br/>Developing a molecular understanding of in vitro and in planta shoot organogenesis, L. Meng, S. Zhang and P.G. Lemaux<br/>Laboratory Exercise: Direct shoot organogenesis from chrysanthemum and african violet leaves, R.N. Trigiano, L.M. Vito, M.T. Windham, S. Boggus, and D. Hadziabdic<br/>Propagation from nonmeristematic tissues - nonzygotic embryogenesis, D.J. Gray<br/>Developmental and molecular aspects of nonzygotic (somatic) embryogenesis, X. Yang and X. Zhang<br/>Laboratory Exercise: Embryogenic callus and suspension cultures from leaves of orchardgrass, D.J. Gray, R.N. Trigiano and B.V. Conger<br/>Laboratory Exercise: Direct non-zygotic embryogenesis from leaves of cineraria, R.N. Trigiano, M.C. Scott, and K.R. Malueg<br/><br/>CROP IMPROVEMENT TECHNIQUES<br/>Protoplasts – an increasingly valuable tool in plant research, J.W. Grosser and A.A. Omar<br/>Laboratory Exercise: Demonstration of principles of protoplast isolation using chrysanthemum and orchardgrass leaves, R.N. Trigiano<br/>Laboratory Exercise: Isolation, culture and fusion of tobacco and potato protoplasts, R.E. Veilleux and M.E. Compton<br/>Haploid cultures, D. Hadziabdic, P.A. Wadl and S.M. Reed<br/>Laboratory Exercise: Haploid plants from tobacco and potato anthers, P.A. Wadl, D. Hadziabdic and S.M. Reed<br/>Concept/Laboratory Exercise: Embryo rescue, T. Eeckhaut , K. Van Laere and J. Van Huylenbroeck<br/>Promoters and gene expression regulation, Z.T. Li and D.J. Gray<br/>Genetic engineering technologies, Z.T. Li, S.A. Dhekney and D.J. Gray<br/>Concept/Laboratory Exercise: Transformation of plant meristems, J.H. Gould<br/>Laboratory Exercise: Genetic transformation of chysanthemum and tobacco using Agrobacterium tumefaciens, M.M. Young, L. Padegimas, N.A. Reichert and R.N. Trigiano<br/>Laboratory Exercise: Genetic transformation of tobacco and production of transgenic plants, S.A. Dhekney, Z.T. Li and D.J. Gray<br/>Genetically modified plant controversies: Sensational headlines versus pragmatic research, H.A. Richards, L.C. Hudson, M.D. Halfhill and C.N. Stewart, Jr.<br/>Cryopreservation of plant cells, tissues and organs, B.M. Reed, M.N. Normah and S.V. Kushnarenko<br/>Laboratory Exercise: Vitrification as a method to cryopreserve shoot tips, S.V. Kushnarenko, B.M. Reed and M.N. Normah<br/>Laboratory Exercise: Cryopreservation of orthodox and recalcitrant seed, M.N. Normah, W.K. Choo, S.V. Kushnarenko and B. Reed<br/>Plant Biotechnology for the Production of Natural Products, A. Kirakosyan, E. M. Seymour and P. Kaufman<br/>Laboratory Exercise: Pigment production in Ajuga cell culture, M.A. Lila and R.B. Rogers<br/>Variations in tissue culture, R.M. Skirvin and M. Norton<br/><br/>THE BUSINESS OF BIOTECHNOLOGY<br/>Biotechnology entrepreneurship in the 21st century: From bench to bag, D.W. Altman<br/>Intellectual property protection for plants, C. Eisenshank |
